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Stability/Storage: Elastase is unstable at pH ≤ 3.5. When stored as a dry powder the enzyme is stable for 6-12 months at 2-8°C. Elastase product codes: ES and ESL have poor solubility at neutral pH and at concentrations greater than 0.25%. It is helpful to make primary solutions in KCl or alkaline buffers before diluting into the reaction mixtures or media, compensating for ionic strength or pH changes. Stable at pH 4.0-10.4.
Technical Notes: 1 SucAla3NA unit is approximately equivalent to 6 elastin digestion units. Aqueous liquid suspensions should be aseptically handled to avoid bacterial contamination. Due to the viscous nature of the aqueous suspension (Code: ES) the vial should be rinsed to recover contents.
Unit Definition: One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.
艾美捷Worthington弹性蛋白酶的应用:
组织解离:因为弹性蛋白在结缔组织的弹性纤维中的浓度高,所以经常使用弹性蛋白酶解离含有广泛细胞间纤维网络的组织。为此,它通常与其他酶起使用,例如胶原酶、胰蛋白酶和胰凝乳蛋白酶。
弹性蛋白酶相关研究:
胶原酶 (CLS1-4/CLSPA)
脱氧核糖核酸酶 I (DP/D/DCLS/D2/DPFF/DPRF)
透明质酸酶 (HSE/HSEP)
中性蛋白酶(分散酶,NPRO)
木瓜蛋白酶 (PAP/PAPL/PAP2)
胃蛋白酶 (PM)
蛋白酶 K (PROK)
胰蛋白酶 (TL/TRL/TRL3/TRLS/TRTPCK)
胰蛋白酶抑制剂 (LBI/OI/SI/SIC)
细胞分离优化系统 (CIT)
肝细胞分离系统 (HIS)
新生儿心肌细胞分离系统 (NCIS)
木瓜蛋白酶解离系统 (PDS/PDS2)