【文献标题】Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress 【作者】J. LIN, J.P. LI, F. YUAN,et.al 【作者单位】山东师范大学（Shandong Normal University） 【文献中引用产品】 植物镁螯合酶(MgCh) ELISA试剂盒（http://www.ayijx.com/c149475/products/d4343206.html） 植物Rubisco活化酶(RCA)ELISA试剂盒（http://www.ayijx.com/c149475/products/d4343207.html） 【关键词】biomass; greening; ion concentrations; photosynthetic parameters; plant height. 【DOI】https://doi.org/10.1007/s11099-018-0824-6 【影响因子(IF)】2.3 【出版期刊】《PHOTOSYNTHETICA》 【产品原文引用】 Protein content ａnd activity of magnesium chelatase ａnd Rubisco: Magnesium chelatase ａnd Rubisco were prepared by first freezing a 0.2-g leaf sample in liquid nitrogen to prevent proteolytic activity, followed by grinding with 2 mL of extraction buffer (100 mM phosphate buffer, pH 7.2, containing 0.1 mM EDTA, ａnd 2 mM ascorbic acid). The homogenate was centrifuged for 15 min at 10,000 rpm, ａnd the supernatant was used to measure the protein content (ng L–1), magnesium chelatase activity [pmol(Mg-Deutero) min–1 mg–1(protein)] ａnd Rubisco activity [μmol min–1 mg–1(protein)]. Magnesium chelatase ａnd Rubisco were measured using a magnesium chelatase content kit ａnd a Rubisco content kit (double antibody sandwich method, Huding Biological Technology Co., Shanghai, China) (3 replicates per treatment).
